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Early Diagnosis Of Many Diseases Simply With Nanoparticles

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Most people think of hydrogen peroxide as a topical germ killer, but the medicine cabinet staple is gaining steam in the medical community as an early indicator of disease in the body


The nanoparticle polymer is made of peroxalate esters. A fluorescent dye (pentacene) is then encapsulated into the polymer. When the nano particles bump into hydrogen peroxide, they excite the dye, which then emits photons (or light) that can be detected (Credit: Image courtesy of Georgia Institute of Technology)

Georgia Institute of Technology and Emory University researchers are the first to create a nanoparticle capable of detecting and imaging trace amounts of hydrogen peroxide in animals. The nanoparticles, thought to be completely nontoxic, could some day be used as a simple, all-purpose diagnostic tool to detect the earliest stages of any disease that involves chronic inflammation — everything from cancer and Alzheimer’s to heart disease and arthritis.

The research, lead by the laboratories of Niren Murthy at the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University and Dr. Robert Taylor in the Division of Cardiology at the Emory University School of Medicine, will appear in the October issue of Nature Materials and was funded by the National Science Foundation (NSF) and the National Institutes of Health (NIH).

Hydrogen peroxide is thought to be over-produced by cells at the early stages of most diseases. Because there were previously no imaging techniques available to capture this process in the body, the details of how the hydrogen peroxide is produced and its role in a developing disease must still be determined.

The Georgia Tech and Emory nanoparticles may be the key to better understanding the role of hydrogen peroxide in the progression of many diseases and later play an important diagnostic role, Murthy said.

“These nanoparticles are incredibly sensitive so you can detect nanomolar concentrations of hydrogen peroxide. That’s important because researchers aren’t yet certain what amounts of hydrogen peroxide are present in various diseases,” Murthy said.

The ultimate goal, however, is that the nanoparticles could some day be used as a simple, all-purpose diagnostic tool for most diseases. In the future, the nanoparticle would be injected by needle into a certain area of the body (for instance, the heart). If the nanoparticles encountered hydrogen peroxide, they would emit light. Should a doctor see a significant amount of light activity in the area, the doctor would know that the patient may be presenting early signs of a disease in that area of the body.

The Georgia Tech and Emory nanoparticles penetrate deep tissue and operate at a high wave length, making them sensitive indicators of the presence of hydrogen peroxide produced by any sort of inflammation.

The nanoparticle polymer is made of peroxalate esters. A fluorescent dye (pentacene) is then encapsulated into the polymer. When the nano particles bump into hydrogen peroxide, they excite the dye, which then emits photons (or light) that can be detected in a simple, photon-counting scan.

“It’s using this nanoparticle made of peroxalate esters that allows you to do this three component reaction in vivo. If you were to inject a peroxalate ester and a dye, they would go their own ways once in the body. With the nanoparticles, we can sequester both of these reagents within nanometers of each other, in vivo,” Murthy said.

The goal was to maximize the wavelength of the particles. Wavelength determines the sensitivity in vivo. And if the particle’s wavelength is high enough, it can penetrate the skin and display clearly on a scan.

The research team started with a nanoparticle that was made of dye and filled with peroxide esthers. They later realized that the reverse (a particle made of peroxalate esters and filled with dye) was more effective at imaging hydrogen peroxide, Murthy said.

The group will conduct further tests with the nanoparticles to confirm their safety and effectiveness.

Nat Mater. 2007 Aug 19; [Epub ahead of print]

In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles.

Lee D, Khaja S, Velasquez-Castano JC, Dasari M, Sun C, Petros J, Taylor WR, Murthy N.

[1] The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA [2] These authors contributed equally to this work.

The overproduction of hydrogen peroxide is implicated in the development of numerous diseases and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalate nanoparticles image hydrogen peroxide by undergoing a three-component chemiluminescent reaction between hydrogen peroxide, peroxalate esters and fluorescent dyes. The peroxalate nanoparticles have several attractive properties for in vivo imaging, such as tunable wavelength emission (460-630 nm), nanomolar sensitivity for hydrogen peroxide and excellent specificity for hydrogen peroxide over other reactive oxygen species. The peroxalate nanoparticles were capable of imaging hydrogen peroxide in the peritoneal cavity of mice during a lipopolysaccharide-induced inflammatory response. We anticipate numerous applications of peroxalate nanoparticles for in vivo imaging of hydrogen peroxide, given their high specificity and sensitivity and deep-tissue-imaging capability.

PMID: 17704780 [PubMed – as supplied by publisher]

Written by huehueteotl

August 24, 2007 at 8:52 am

3 Responses

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  1. What is a good, concise definition for peroxalate?

    I read the article “Nanoparticle Could Help Detect Diseases Early” in Georgia Tech’s “Buzzwords” and chose to include it in my Medical Terminology course’s article project. I have to take 10 medical terms from the article, define them, and use them in a sentence. I’ve done so for every word but peroxalate. It is difficult to find a definition for this word. Could someone help me define peroxalate and maybe even give a sample sentence using this word? Thank you!

    Robyn
    Amazable@aol.com

    Robyn

    September 7, 2007 at 9:25 pm

  2. Hey Robin,

    Peroxalates are the salts of peroxalic acid. Oxalic acid is (COOH)2.
    Therefore peroxalic acid would be (COOOH)2. It is rather a chemical, than a truely medical term though.

    Peroxalic systems are used for photoluminescence:
    http://en.wikipedia.org/wiki/Photoluminescence

    Here they use esters – formally formed in a condensation reaction between an alcohol and an acid in a reaction known as esterification. The most common type of esters are carboxylic acid esters (R1-C(=O)OR2), but other acids also form analogous esters. The the peroxalic acid even would be one of them. http://en.wikipedia.org/wiki/Ester
    Good luck with your project!

    Dirk
    huehueteotl

    huehueteotl

    September 7, 2007 at 9:51 pm

  3. Not that I’m totally impressed, but this is a lot more than I expected for when I found a link on SU telling that the info here is awesome. Thanks.

    How to Get Six Pack Fast

    April 15, 2009 at 4:07 pm


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